2Z4G

Histidinol Phosphate Phosphatase from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-03-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	84.961, 96.786, 74.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 1.800
R-factor	0.224
Rwork	0.224
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yxo
RMSD bond length	0.019
RMSD bond angle	1.900
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.540
High resolution limit [Å]	1.800
Number of reflections	57365
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M Tris-HCl pH8.5, 0.68M Sodium Malonate, VAPOR DIFFUSION, HANGING DROP, temperature 293K