2Z46
Crystal Structure of Native-ORF134
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.794, 93.794, 410.222 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.970 |
| R-factor | 0.24961 |
| Rwork | 0.248 |
| R-free | 0.27675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z44 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.088 | 0.726 |
| Number of reflections | 37069 | |
| Completeness [%] | 99.3 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.4M Na acetate, 0.1M Na cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
