2Z44
Crystal Structure of Selenomethionine-labeled ORF134
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97923, 0.97946, 0.96423 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 56.461, 56.461, 85.167 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.800 - 2.500 |
| R-factor | 0.20823 |
| Rwork | 0.205 |
| R-free | 0.28050 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.463 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.109 | 0.461 |
| Number of reflections | 5247 | |
| Completeness [%] | 98.7 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50% PEG 200, 0.1M phosphate-citrate pH 4.2, 0.2M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
