2Z41
Crystal Structure Analysis of the Ski2-type RNA helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 133.737, 133.737, 133.636 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.510 |
| Rwork | 0.291 |
| R-free | 0.34380 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.162 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.510 | 3.510 |
| Rmerge | 0.129 | 0.454 |
| Number of reflections | 16159 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 10% 2-propanol, 0.2M Lithium sulfate, 0.1M phosphate-citrate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
