2Z3X
Structure of a Protein-DNA Complex Essential for DNA Protection in Spore of Bacillus Species
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 86.962, 86.962, 144.669 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.930 - 2.100 |
R-factor | 0.257 |
Rwork | 0.257 |
R-free | 0.28300 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.050 | 0.292 |
Number of reflections | 17711 | |
<I/σ(I)> | 15.9 | 2.7 |
Completeness [%] | 90.5 | 59.4 |
Redundancy | 7.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 0.01M magnesium sulfate, 0.05M sodium cacodylate, 1.4M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 0.01M magnesium sulfate, 0.05M sodium cacodylate, 1.4M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | magnesium sulfate | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | ammonium sulfate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | magnesium sulfate | ||
6 | 1 | 2 | odium cacodylate | ||
7 | 1 | 2 | ammonium sulfate | ||
8 | 1 | 2 | H2O |