2Z3W
Prolyl tripeptidyl aminopeptidase mutant E636A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 149.709, 149.709, 160.943 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.18753 |
Rwork | 0.186 |
R-free | 0.21609 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2d5l |
RMSD bond length | 0.016 |
RMSD bond angle | 1.449 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 0.314 |
Number of reflections | 71953 | |
<I/σ(I)> | 81 | 11.6 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 20 | 19.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.1M potassium sodium tartrate, 0.2M lithium sulfate, 0.1M CHES buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |