2Z3V
Crystal structure of uncharacterized conserved protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-11 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.064, 73.064, 57.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.280 - 1.650 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wjg |
| RMSD bond length | 0.038 |
| RMSD bond angle | 2.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.048 | 0.340 |
| Number of reflections | 19483 | |
| <I/σ(I)> | 60.1 | 9.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.4 | 20.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 40% v/v 1,2-propanediol, 0.1M sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
