2Z3T
Crystal Structure of Substrate Free Cytochrome P450 StaP (CYP245A1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-09-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.640, 78.718, 145.656 |
| Unit cell angles | 90.00, 107.92, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.228 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.536 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 138934 | |
| <I/σ(I)> | 20.66 | 3.34 |
| Completeness [%] | 91.4 | 67.1 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 22% PEG 3350, 50mM potassium fluoride, 100mM imidazole, pH 8.0, VAPOR DIFFUSION, temperature 293K |
| 1 | VAPOR DIFFUSION | 8 | 293 | 22% PEG 3350, 50mM potassium fluoride, 100mM imidazole, pH 8.0, VAPOR DIFFUSION, temperature 293K |
