2Z3A
Crystal Structure of Bacillus Subtilis CodW, a non-canonical HslV-like peptidase with an impaired catalytic apparatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-26 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.130, 164.420, 95.900 |
| Unit cell angles | 90.00, 111.53, 90.00 |
Refinement procedure
| Resolution | 40.370 - 3.000 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.117 | |
| Number of reflections | 44150 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 98.1 | 97.6 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | MPD, SODIUM CITRATE, GLYCEROL, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
