2Z34
Crystal structure of SpCia1/Asf1 complex with Hip1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.013, 48.086, 63.247 |
| Unit cell angles | 90.00, 99.25, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.19756 |
| Rwork | 0.194 |
| R-free | 0.25408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cu9 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.739 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | 0.237 |
| Number of reflections | 16188 | |
| Completeness [%] | 97.6 | 98.7 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 25% PEG3350, 0.18M Ammonium Fluoride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
