2Z2X
Crystal structure of mature form of Tk-subtilisin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.055, 60.958, 91.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.620 - 1.700 |
| R-factor | 0.14002 |
| Rwork | 0.139 |
| R-free | 0.16025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e1p |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.054 | 0.222 |
| Number of reflections | 29017 | |
| <I/σ(I)> | 46.8 | 7.6 |
| Completeness [%] | 99.5 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.1M Sodium Acetate trihydrate, 0.2M Ammonium Sulfate, 25% (w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
