2Z2S
Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2003-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97969 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.135, 46.452, 141.701 |
| Unit cell angles | 90.00, 91.63, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.2865 |
| Rwork | 0.250 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | 0.079 |
| Number of reflections | 24975 | |
| <I/σ(I)> | 9.8 | 2.14 |
| Completeness [%] | 75.8 | 60.7 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295.15 | 0.1 M MES, 0.2 M ammonium sulfate, 14-18% polyethylene glycol monomethylether 5000 (PEG5KMME), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
