2Z2R
Nucleosome assembly proteins I (NAP-1, 74-365)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 1.1 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 125.000, 125.000, 146.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.200 |
Rwork | 0.260 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ayu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.537 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.070 | 0.380 |
Number of reflections | 17067 | |
<I/σ(I)> | 14 | 3.5 |
Completeness [%] | 86.4 | 92 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 40% MPD, 0.1M sodium citrate, 0.2M ammonium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |