2Z2L
Penicillin-Binding Protein 2X (PBP2X) from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32B2 |
Synchrotron site | SPring-8 |
Beamline | BL32B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-18 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 106.881, 171.710, 89.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.850 |
R-factor | 0.22686 |
Rwork | 0.224 |
R-free | 0.28568 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qme |
RMSD bond length | 0.007 |
RMSD bond angle | 1.067 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.061 | 0.232 |
Number of reflections | 39032 | |
<I/σ(I)> | 17.1 | 5.7 |
Completeness [%] | 99.8 | 0.998 |
Redundancy | 5.7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10-25% PEG4000, 0.2M ammonium sulfate, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP |