2Z2K

Crystal structure of Peptidyl-tRNA hydrolase from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-03-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	35.840, 57.060, 72.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.010 - 2.500
R-factor	0.198
Rwork	0.198
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pth
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.580
High resolution limit [Å]	2.490	2.490
Number of reflections	5428
<I/σ(I)>	15.14	3.6
Completeness [%]	98.2	95
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	298	15%(w/v) PEG 8000, 100mm HEPES, 5%(v/v) 2-propanol, pH 7.5, Microbatch, temperature 298K