2Z2F

X-ray Crystal Structure of Bovine Stomach Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2006-05-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	31.257, 56.065, 64.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.940 - 1.500
R-factor	0.18
Rwork	0.178
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lz1
RMSD bond length	0.009
RMSD bond angle	1.261
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	17833
<I/σ(I)>	36.374	17.272
Completeness [%]	95.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K