2Z2E
Crystal Structure of Canine Milk Lysozyme Stabilized against Non-enzymatic Deamidation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 |
| Unit cell lengths | 31.216, 31.216, 198.307 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.040 - 2.010 |
| R-factor | 0.2795 |
| Rwork | 0.273 |
| R-free | 0.41266 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cwi |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.774 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 14193 | |
| <I/σ(I)> | 25.927 | 11.992 |
| Completeness [%] | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 10mM sodium citrate, 1M ammonium sulfate, 5% ethylene glycol, 2.5% iso-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
