2Z2A
Thr109Gly dihydroorotase from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-30 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.435, 78.193, 179.961 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.870 |
R-factor | 0.15454 |
Rwork | 0.152 |
R-free | 0.19612 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xge |
RMSD bond length | 0.013 |
RMSD bond angle | 1.443 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.920 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.048 | 0.152 |
Number of reflections | 56036 | |
<I/σ(I)> | 34.1 | 8.7 |
Completeness [%] | 91.9 | 91.7 |
Redundancy | 4.6 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 277 | 17% PEG 3350, 0.1M MES, 75mM MgCl2, 0.15M KCl, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |