2Z28
Thr109Val dihydroorotase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.378, 78.413, 179.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.870 |
| R-factor | 0.15606 |
| Rwork | 0.154 |
| R-free | 0.18910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xge |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.398 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.064 | 0.189 |
| Number of reflections | 58553 | |
| <I/σ(I)> | 22.7 | 6.4 |
| Completeness [%] | 95.9 | 97.8 |
| Redundancy | 4.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 277 | 17% PEG3350, 0.1M MES, 75mM MgCl2, 0.15M KCl, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
