2Z1H
Crystal structure of E.coli RNase HI surface charged mutant(Q4R/T92K/Q105K/Q113R/Q115K/N143K/T145K)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-03 |
Detector | BRUKER SMART 6500 |
Wavelength(s) | 0.9 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 88.206, 88.206, 55.693 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.580 - 2.600 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rn2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.032 | 0.180 |
Number of reflections | 8920 | |
<I/σ(I)> | 20 | 3.9 |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100mM HEPES-NaOH, 15-25% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |