2Z1C
Crystal structure of HypC from Thermococcus kodakaraensis KOD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.237, 59.130, 53.973 |
| Unit cell angles | 90.00, 109.01, 90.00 |
Refinement procedure
| Resolution | 46.180 - 1.800 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.24600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 21035 | |
| <I/σ(I)> | 31.3 | 4.3 |
| Completeness [%] | 97.3 | 93.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1M citrate acid, pH 4.3-4.5, 100-600mM NaBr, 17-20% PEG4000, 20% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
