2Z19
Phase transition of monoclinic lysozyme crystal soaked in a saturated NaCl solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-12-28 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.085, 59.055, 30.703 |
| Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.150 |
| Rwork | 0.178 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d4j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.027 |
| Data reduction software | SAINT (Plus) |
| Data scaling software | SAINT (Plus) |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.088 | 0.211 |
| Number of reflections | 29674 | |
| Completeness [%] | 93.2 | 72.6 |
| Redundancy | 4.23 | 2.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Dehydration-induced phase transition | 7.6 | 293 | NaCl, nitrogen-gas, pH 7.6, Dehydration-induced phase transition, temperature 293K |
