2Z18
Phase transition of monoclinic lysozyme crystal soaked in a 10% NaCl solution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-12-11 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.622, 60.588, 31.223 |
Unit cell angles | 90.00, 111.44, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.150 |
Rwork | 0.156 |
R-free | 0.17600 |
Structure solution method | SHELX-97 |
Starting model (for MR) | 2d4j |
RMSD bond length | 0.012 |
RMSD bond angle | 0.029 |
Data reduction software | SAINT (Plus) |
Data scaling software | SAINT (Plus) |
Phasing software | SHELXL-97 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.104 | 0.090 |
Number of reflections | 32729 | |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 4.73 | 3.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Dehydration induced phase transition | 293 | 10% NaCl, temperature-regulated nitrogen gas, Dehydration induced phase transition, temperature 293K |