2Z17
Crystal structure of PDZ domain from human Pleckstrin homology, Sec7
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9789, 0.9794, 0.964 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 65.917, 65.917, 81.090 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.540 - 2.700 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.33400 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.630 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 3931 | |
<I/σ(I)> | 41.9 | 15.6 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 19.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES-Na, 2.0M Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |