2Z16
Crystal structure of Matrix protein 1 from influenza A virus A/crow/Kyoto/T1/2004(H5N1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-04 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.079, 63.909, 55.742 |
| Unit cell angles | 90.00, 95.11, 90.00 |
Refinement procedure
| Resolution | 41.920 - 2.020 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aa7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.887 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 17580 | |
| <I/σ(I)> | 33.8 | 4.72 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3.64 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris-HCl, 0.2M Magnesium Chloride, 30 % PEG 4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
