2Z15

Crystal structure of human Tob1 protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-06
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9789, 0.9794, 0.964
Spacegroup name	P 21 21 21
Unit cell lengths	36.064, 121.162, 152.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.440 - 2.300
R-factor	0.194
Rwork	0.194
R-free	0.24600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	30540
<I/σ(I)>	21.6	4.94
Completeness [%]	99.9	99.9
Redundancy	6.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	0.1M Sodium acetate trihydrate, 8% PEG4000, pH4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K