2Z11

Crystal structure of putative acetyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-10-28
Detector	RIGAKU RAXIS VII
Wavelength(s)	2.29
Spacegroup name	P 32 2 1
Unit cell lengths	70.956, 70.956, 99.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.400 - 2.150
Rwork	0.204
R-free	0.23200
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	16175
<I/σ(I)>	35	9.6
Completeness [%]	99.8	98.4
Redundancy	17.6	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.15M ammonium acetate, 0.1M bis-tris(5.5), 28% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K