2Z0X
Crystal structure of ProX-CysSA complex from T. thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-28 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.512, 50.613, 81.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.750 - 1.640 |
Rwork | 0.158 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cx5 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Number of reflections | 16242 | |
<I/σ(I)> | 59 | 11.6 |
Completeness [%] | 96.9 | 77.3 |
Redundancy | 6.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG 3350, 0.1M Hepes, 30mM CysSA, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |