2Z0V

Crystal structure of BAR domain of Endophilin-III

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97931, 0.97965, 0.9640
Spacegroup name	P 21 21 2
Unit cell lengths	56.818, 194.167, 43.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.040 - 2.490
R-factor	0.242
Rwork	0.242
R-free	0.32200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.490	2.490
Number of reflections	16765
<I/σ(I)>	11.44
Completeness [%]	94.6	89.2
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Na Formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K