2Z0N

Crystal structure of APPL1-BAR domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.979089, 0.979462, 0.964
Spacegroup name	P 21 21 2
Unit cell lengths	54.121, 128.329, 36.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.520 - 1.950
R-factor	0.209
Rwork	0.209
R-free	0.25900
Structure solution method	MAD
RMSD bond length	0.024
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	19533
<I/σ(I)>	16.8
Completeness [%]	99.9	100
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	17.5% PEG3350, 0.2M Ammonium sulfate, 0.1M Hepes, VAPOR DIFFUSION, HANGING DROP, temperature 293K