2Z0L

Crystal structure of EBV-DNA polymerase accessory protein BMRF1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-25
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97907, 0.97947, 0.96400
Spacegroup name	C 2 2 21
Unit cell lengths	125.671, 191.635, 371.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.380 - 2.900
R-factor	0.206
Rwork	0.206
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.390	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	98550
<I/σ(I)>	15.4
Completeness [%]	99.6	100
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.25M Li sulfate, 0.5M Ammonium sulfate, 8% PEG 400, 0.1M Hepes pH7.5, 27.5mM 2,6-dimethyl-4-heptyl-B-D-maltoside, VAPOR DIFFUSION, SITTING DROP, temperature 293K