2Z0A
Crystal structure of RNA-binding domain of NS1 from influenza A virus A/crow/Kyoto/T1/2004(H5N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-13 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.723, 54.378, 66.347 |
Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.850 |
R-factor | 0.17584 |
Rwork | 0.172 |
R-free | 0.23828 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ail |
RMSD bond length | 0.008 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 22185 | |
<I/σ(I)> | 17.4 | 2 |
Completeness [%] | 97.8 | 90.9 |
Redundancy | 4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 0.1M SPG buffer pH 6.8, 25 % (w/v) PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 293K |