2Z0A
Crystal structure of RNA-binding domain of NS1 from influenza A virus A/crow/Kyoto/T1/2004(H5N1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.723, 54.378, 66.347 |
| Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.17584 |
| Rwork | 0.172 |
| R-free | 0.23828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ail |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 22185 | |
| <I/σ(I)> | 17.4 | 2 |
| Completeness [%] | 97.8 | 90.9 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 0.1M SPG buffer pH 6.8, 25 % (w/v) PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
