2Z09
Crystal structure of uncharacterized conserved protein from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-02 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 33.220, 75.900, 88.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.220 - 1.650 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wjg |
RMSD bond length | 0.029 |
RMSD bond angle | 2.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.670 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.048 | 0.200 |
Number of reflections | 13263 | |
<I/σ(I)> | 30.9 | 7.82 |
Completeness [%] | 95.3 | 100 |
Redundancy | 6.5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 10mM AMP-PCP, 100mM Magnesium Chloride, 0.09M Sodium acetate trihydrate, 1.8M Sodium chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |