2Z07

Crystal structure of uncharacterized conserved protein from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-09
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9789, 0.9000, 0.9794
Spacegroup name	H 3
Unit cell lengths	93.437, 93.437, 254.918
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.980 - 2.100
R-factor	0.189
Rwork	0.189
R-free	0.23300
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.059	0.260
<I/σ(I)>	17.2	4.39
Completeness [%]	99.8	100
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	293	29% v/v MPD, 0.1M Sodium citrate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K