2Z04

Crystal structure of phosphoribosylaminoimidazole carboxylase ATPase subunit from Aquifex aeolicus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-06
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9789, 0.9793, 0.9000
Spacegroup name	P 1
Unit cell lengths	51.291, 55.053, 74.541
Unit cell angles	93.93, 101.54, 114.23

Refinement procedure

Resolution	32.410 - 2.350
R-factor	0.232
Rwork	0.232
R-free	0.26700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.410	2.500
High resolution limit [Å]	2.350	2.350
Number of reflections	56281
Completeness [%]	94.0	86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	47.5% PEG200, CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K