2YZS

Crystal structure of uncharacterized conserved protein from Aquifex aeolicus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-01
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9788, 0.9000, 0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	122.235, 96.979, 93.495
Unit cell angles	90.00, 127.34, 90.00

Refinement procedure

Resolution	40.610 - 2.000
R-factor	0.219
Rwork	0.219
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.055	0.302
Number of reflections	57566
<I/σ(I)>	15.7	3.8
Completeness [%]	99.1	97.2
Redundancy	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.7M Ammonium sulfate, 85MM HEPES pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K