2YZJ
Crystal structure of dCTP deaminase from Sulfolobus tokodaii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-09 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9789, 0.9794, 0.9000 |
Spacegroup name | P 1 |
Unit cell lengths | 53.770, 53.757, 53.757 |
Unit cell angles | 107.47, 107.52, 107.50 |
Refinement procedure
Resolution | 31.650 - 1.660 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.121 | 0.236 |
Number of reflections | 90293 | |
Completeness [%] | 92.0 | |
Redundancy | 2.7 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.17M Magnesium Acetate, 0.085M Sodium Cavpdylate pH 6.5, 17% PEG 8000, 0.01M dUTP, 0.01M Magnesium Chloride, 0.02M Tris-HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |