2YZJ
Crystal structure of dCTP deaminase from Sulfolobus tokodaii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-09 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9789, 0.9794, 0.9000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.770, 53.757, 53.757 |
| Unit cell angles | 107.47, 107.52, 107.50 |
Refinement procedure
| Resolution | 31.650 - 1.660 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.121 | 0.236 |
| Number of reflections | 90293 | |
| Completeness [%] | 92.0 | |
| Redundancy | 2.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.17M Magnesium Acetate, 0.085M Sodium Cavpdylate pH 6.5, 17% PEG 8000, 0.01M dUTP, 0.01M Magnesium Chloride, 0.02M Tris-HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
