2YZI
Crystal structure of uncharacterized conserved protein from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-01 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9792, 0.9797, 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.495, 55.499, 97.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.200 - 2.250 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.24100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.018 | 0.321 |
| Number of reflections | 24075 | |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2M Sodium Chloride, 0.1M Bis-Tris pH 5.5, 25% PEG 3350 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
