2YZE

Crystal structure of uricase from Arthrobacter globiformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	85.430, 122.540, 284.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.990
Rwork	0.191
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1UOX
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.220	2.060
High resolution limit [Å]	1.990	1.990
Rmerge	0.060	0.177
Number of reflections	193433
<I/σ(I)>		4.7
Completeness [%]	91.8	87.1
Redundancy	3.47	3.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	PEG 8000, lithium sulfate, sodium borate buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K