2YZ7
X-ray analyses of 3-hydroxybutyrate dehydrogenase from Alcaligenes faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.302, 118.827, 118.726 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.190 |
| Rwork | 0.197 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hsd |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.190 | 2.200 |
| Rmerge | 0.076 | 0.340 |
| Number of reflections | 102135 | |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 28% PEG 4000, 0.1M lithium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
