2YYZ
Crystal structure of Sugar ABC transporter, ATP-binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 120 |
Detector technology | CCD |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.063, 86.774, 105.681 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.120 - 2.110 |
R-factor | 0.22622 |
Rwork | 0.223 |
R-free | 0.27973 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d62 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.386 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.120 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.302 |
Number of reflections | 24616 | |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2M Li Sulfate, 21% PEG3350, 0.1M HEPES, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |