2YYV

Crystal structure of uncharacterized conserved protein from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-13
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	68.585, 72.414, 178.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.600 - 1.650
R-factor	0.221
Rwork	0.221
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Rmerge	0.039
Number of reflections	52400
Completeness [%]	97.7
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES pH7.5, 10% iso-Propanol, 20% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K