2YYR
Structural analysis of PHD domain of Pygopus complexed with trimethylated histone H3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.518, 59.518, 95.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.430 - 2.500 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dx8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.094 | 0.315 |
| Number of reflections | 6470 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.9 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | co-crystallization | 9 | 298 | 1.0M Na Citrate, 0.1M LiSo4, 0.1mM ZnCl2, 50mM Tris-HCl, pH 9.0, co-crystallization, temperature 298K |
