2YYO

Crystal structure of human SPRY domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9789, 0.9793, 0.971
Spacegroup name	P 43 21 2
Unit cell lengths	52.107, 52.107, 108.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.050 - 2.000
R-factor	0.218
Rwork	0.218
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	18943
<I/σ(I)>	78.6	20.4
Completeness [%]	100.0	100
Redundancy	24.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1M Tris, 1.6M Ammonium Sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K