2YY8
Crystal structure of archaeal tRNA-methylase for position 56 (aTrm56) from Pyrococcus horikoshii, complexed with S-adenosyl-L-methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 |
Unit cell lengths | 56.151, 56.151, 121.844 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.170 - 2.480 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.064 | 0.274 |
Number of reflections | 15052 | |
<I/σ(I)> | 27.1 | 4.9 |
Completeness [%] | 98.2 | 92.8 |
Redundancy | 4.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 20% PEG3000 100mM Acetate (pH4.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 20% PEG3000 100mM Acetate (pH4.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |