2YY7

Crystal structure of thermolabile L-threonine dehydrogenase from Flavobacterium frigidimaris KUC-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	61.029, 88.142, 123.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.474 - 2.061
Rwork	0.195
R-free	0.20300
Structure solution method	MIRAS
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	88.045
High resolution limit [Å]	2.061
Number of reflections	44410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	32% PEG 600, 5% PEG 1000, 10% glycerol, 0.1M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K