2YY3

Crystal Structure of translation elongation factor EF-1 beta from Pyrococcus horikoshii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-08
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97874, 0.97904, 0.99476
Spacegroup name	P 21 21 21
Unit cell lengths	50.001, 77.791, 114.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.180 - 2.500
R-factor	0.236
Rwork	0.236
R-free	0.28500
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	32040
Completeness [%]	99.7	99.9
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	293	0.1M Tris-HCl, 0.2M Lithium Sulfate, 2M Ammonium Sulfate, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K