2YY1

Crystal structure of N-terminal domain of human galectin-9 containing L-acetyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-17
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	47.407, 47.407, 121.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.810 - 2.170
R-factor	0.211
Rwork	0.211
R-free	0.24400
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.150	2.150
Number of reflections	15074
<I/σ(I)>	46.3176	11.3
Completeness [%]	97.1	78.8
Redundancy	12.8989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	0.2M sodium acetate trihydrate, 0.1M sodium cacodylate, 30% PEG 8000, 30mM L-acetyllactosamine, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K