2YY0

Crystal Structure of MS0802, c-Myc-1 binding protein domain from Homo sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9790
Spacegroup name	H 3
Unit cell lengths	59.634, 59.634, 194.868
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.110 - 2.400
R-factor	0.244
Rwork	0.244
R-free	0.28900
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	10097
<I/σ(I)>	21.63	9.83
Completeness [%]	99.5	100
Redundancy	6.02	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.1M Tris-HCl, 2M NH4H2PO4, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K