2YXZ
Crystal structure of tt0281 from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.564, 52.190, 181.124 |
| Unit cell angles | 90.00, 108.33, 90.00 |
Refinement procedure
| Resolution | 48.650 - 1.900 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26400 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 171.499 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 91864 | |
| Completeness [%] | 92.2 | 76.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ammonium Sulfate, Dioxane, HEPES-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
