2YXZ
Crystal structure of tt0281 from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.564, 52.190, 181.124 |
Unit cell angles | 90.00, 108.33, 90.00 |
Refinement procedure
Resolution | 48.650 - 1.900 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26400 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 171.499 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 91864 | |
Completeness [%] | 92.2 | 76.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ammonium Sulfate, Dioxane, HEPES-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |